名稱 | TLR3/NFkB-Luc/HEK293 |
型號 | CBP74057 |
報價 | |
特點 | TLR3/NFkB-Luc/HEK293,凍存條件:90% FBS+10% DMSO; |
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- 詳細內容
CBP74057 | |
I. Background | |
TLR3 (Toll Like Receptor 3) is a Protein Coding gene. Diseases associated with TLR3 include Encephalopathy, Acute, Infection-Induced 2 and Human Immunodeficiency Virus Type 1. Among its related pathways are Activated TLR4 signalling and Toll-like Receptor Signaling Pathway. Gene Ontology (GO) annotations related to this gene include identical protein binding and transmembrane signaling receptor activity. | |
II. Introduction | |
Host Cell: | HEK293 |
Expressed gene: | TLR3、NFkB-Luc |
Stability: | 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
Freeze Medium: | 90% FBS+10% DMSO |
Culture Medium: | MEM+10% Foetal Bovine Serum (FBS)+ 1% Non Essential Amino Acids (NEAA) + 1mM Sodium Pyruvate (NaP) +1ug/ml puromycin+100ug/ml hygromycin |
Mycoplasma Testing: | Negative |
Application(s): | Functional(Report Gene) Assay |
Storage: | Liquid nitrogen |
III. Description of Host Cell Line | |
Organism: | Human |
Tissue: | Embryonic kidney |
Disease: | Normal |
Morphology: | Epitheloid cell |
Growth Properties: | Adherent |
IV. Representative Data | |
Figure 1. Detect Luciferase assay by Ultra Luciferase Detection Kit CBPH0001(we strongly suggest to purchase from Cobioer).TLR3/NF-kB Reporter-HEK293 cells were stimulated by Poly(I:C), the S/B was 57-fold. |