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藥靶細(xì)胞株 > 腫瘤免疫細(xì)胞株 > CBP74060LAG3/NFAT-Luc/Jurkat

LAG3/NFAT-Luc/Jurkat
名稱 LAG3/NFAT-Luc/Jurkat
型號(hào) CBP74060
報(bào)價(jià)
特點(diǎn) LAG3/NFAT-Luc/Jurkat，凍存條件：90% FBS+10% DMSO；
  • 詳細(xì)內(nèi)容


CBP74060
I. Background

Lymphocyte-activation gene 3 (LAG3, CD223) is a cell surface protein that belongs to immunoglobulin (Ig) superfamily. LAG3 is expressed on activated T cells, natural killer cells, B cells, and plasmacytoid dendritic cells.

II. Description
Recombinant Jurkat T cell expressing firefly luciferase gene under the control of NFAT response elements with constitutive expression of human LAG3 (lymphocyte-activation gene 3, CD223, GenBank Accession # NM_002286).
III. Introduction
Host Cell:Jurkat
Expressed gene:

NFAT-LAG3-Luciferase

Stability:32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.)
Synonym(s):LAG3, LAG-3, CD223, Cell Line, Jurkat
Freeze Medium:90% FBS+10% DMSO
Culture Medium:

RPMI-1640+10%FBS+1ug/ml puromycin+400ug/ml hygromycin

Mycoplasma Testing:Negative
Storage:Liquid nitrogen
Application(s):

Functional(Report Gene) Assay

IV. Description of Host Cell Line
Organism:Human
Tissue:Peripheral blood
Disease: Childhood T acute lymphoblastic leukemia
Morphology: Lymphoblast
Growth Properties: Suspension
Ⅴ. Representative Data

COBIOER

Figure 1. Recombinant Jurkat stably expressing human LAG3.

COBIOER

Figure 2. Detect Luciferase assay by Ultra Luciferase Detection Kit CBPH0001（we strongly suggest to purchase from Cobioer). Dose Response of Anti-LAG3 Neutralizing Antibody in LAG3/NFAT Reporter-Jurkat cells.








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