名稱 | mouse ADCC Bioassay Effector Cell Line |
型號 | CBP74076 |
報價 | |
特點 | Mouse ADCC Bioassay Effector Cell Line,凍存條件:90% FBS+10% DMSO |
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mouse ADCC Bioassay Effector Cell Line mouse ADCC Bioassay Effector Cell Line I. Background | |
抗體依賴的細胞介導的細胞毒性作用(ADCC,antibody-dependent cell-mediated cytotoxicity )是指抗體的 Fab 段結(jié)合病毒感染的細胞或腫瘤細胞的抗原表位,其 Fc 段與殺 傷細胞(NK 細胞、巨噬細胞等)表面的 FcR 結(jié)合,介導殺傷細胞直接殺傷靶細胞,它是 抗腫瘤的治療性抗體藥物發(fā)生作用的一種作用重要機制。 | |
II. Description | |
Mouse ADCC Bioassay Effector Cell 報告基因藥靶模型很好的模擬了體內(nèi) ADCC 的信號轉(zhuǎn)導過程,原理見下圖所示。 Figure 1. Mouse ADCC Bioassay Effector Cell 模型原理圖 | |
III. Introduction | |
Host Cell: | Jurkat |
Expressed gene: | FcγRⅣ |
Stability: | 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
Freeze Medium: | 90% FBS+10% DMSO |
Culture Medium: | RPMI-1640+10%FBS+800 μg/ml hygromycin+1 μg/ml puromycin |
Mycoplasma Testing: | Negative |
Storage: | Liquid nitrogen |
Application(s): | Functional(Report Gene) Assay |
IV. Representative Data |
Figure 2. Dose response of Anti-hCD20-mlgG2a in Mouse ADCC Bioassay Effector Cell (C3).