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藥靶細胞株 > kinase激酶細胞株 > CBP73184Her2 A775_G776insYVMA/BaF3

Her2 A775_G776insYVMA/BaF3
名稱 Her2 A775_G776insYVMA/BaF3
型號 CBP73184
報價
特點 Her2 A775_G776insYVMA/BaF3,母細胞:BaF3,凍存條件:90% FBS+10% DMSO
  • 詳細內(nèi)容
CBP73184
I. Introduction

Cell Line Name:

Her2 A775_G776insYVMA/BaF3

Host Cell:

Ba/F3

Stability:16 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.)

Application:

Anti-proliferation assay and PD assay

Freeze Medium:

90% FBS+10% DMSO

Complete Culture Medium:

RPMI-1640+10%FBS

Mycoplasma Status:

Negative


II.Background

ERBB2 (HER2) A775_G776insYVMA results in the insertion of four amino acids in the protein kinase domain of the Erbb2 (Her2) protein between amino acids 775 and 776 (UniProt.org). A775_G776insYVMA results in the same amino acid change as M774_A775insAYVM, which leads to constitutive phosphorylation of Erbb2 (Her2) and is transforming in cell culture (PMID: 17311002, PMID: 29967253).


III. Representative Data

1. WB of Her2 A775_G776insYVMA/BaF3

CBP73184 WB.png


2. Anti-proliferation assay

CBP73184 fig.png

Figure 2. Anti-proliferation assay of two reference compounds on the Her2 A775_G776insYVMA/BaF3 Stable Cell Line




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