名稱 | HVEM/NFκB-Luc/Jurkat |
型號 | CBP74013 |
報價 | |
特點 | HVEM/NFκB-Luc/Jurkat,凍存條件:90% FBS+10% DMSO; |
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I. Background | |
皰疹病毒進入介質(zhì)(HVEM),也稱為腫瘤壞死因子受體超家族成員 14 (TNFRSF14),是 TNF 受體(腫瘤壞死因子)超家族的人細胞表面受體。HVEM 在多種 免疫細胞中表達,包括 T 細胞、B 細胞、自然殺傷細胞、樹突狀細胞和內(nèi)皮細胞。它還通 過在黑色素瘤細胞上的頻繁表達參與腫瘤的免疫逃避。 | |
II. Description | |
HVEM NFκB-Luc Jurkat 報告基因藥靶模型很好的模擬了體內(nèi) HVEM 的信號轉(zhuǎn)導(dǎo)過程, 原理見下圖所示。 Figure 1. HVEM NFκB-Luc Jurkat 細胞模型原理圖 | |
III. Introduction | |
Host Cell: | Jurkat |
Expressed gene: | HVEM-NFκB-Luciferase |
Stability: | 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
Synonym(s): | HVEM, TNFRSF14, CD270, HVEA, TR2, LIGHTR, HVEM Cell line, HVEM NFAT |
Freeze Medium: | 90% FBS+10% DMSO |
Culture Medium: | RPMI-1640+10%FBS+800μg/ml hygromycin+1μg/ml puromycin |
Mycoplasma Testing: | Negative |
Storage: | Liquid nitrogen |
Application(s): | Functional(Report Gene) Assay |
IV. Description of Host Cell Line | |
Organism: | Human |
Tissue: | Peripheral blood |
Disease: | Childhood T acute lymphoblastic leukemia |
Morphology: | Lymphoblast |
Growth Properties: | Suspension |
Ⅴ. Representative Data | |
Figure 2. Recombinant Jurkat stably expressing human HVEM. |
Figure 3. Detect Luciferase assay by Ultra Luciferase Detection Kit CBPH0001(we strongly suggest to purchase from Cobioer). HVEM NFκB-Luc Jurkat(C7) Report Assay Stimulated by LIGHT/CHO.